HAV305V Biochemistry 3 (3 ECTS)

Overview

Description:
This TU allows students to consolidate the fundamentals of biochemistry acquired in their first year by approaching the discipline through a cross-disciplinary study of enzymes involved in cellular metabolism, particularly glycolysis. Several areas of biochemistry will be covered: the fundamentals of Michaelis-Menten kinetics, and a description of the metabolic reactions involved in glycolysis. Finally, the technical aspect will be addressed through the presentation and analysis of techniques used to measure enzymatic activity and to purify, quantify, and detect proteins.

Objectives:

• Understand the basic principles and concepts of Michaelis-Menten kinetics and cellular energetics in order to understand metabolic regulation.

• Basic knowledge of the structure and metabolism of carbohydrates, particularly glycolysis. 

• Understand that the “spontaneity” of the reactions that make up a metabolic pathway in cells is linked to a combination of intracellular factors (variable concentrations of different metabolites over time) and extracellular factors (concentrations of secreted metabolites, hormonal signals, etc.). 

• Detect and quantify enzymatic activity; measure reaction rates. Quantify a metabolite. 

• Understand and apply common spectrophotometric assay and analysis techniques, and master common separation and purification methods. 

• Understand intermolecular interactions.

Hourly volumes:

Lecture: 2:00 PM
Tutorial: 2:00 PM
Lab: 8:00 AM
Fieldwork: 0 hours

Required prerequisites:

Bachelor's degree in science or health sciences.

Recommended prerequisites:

No changes

More information

Assessment:

1 tutorial exam in the lecture hall (10%)

Final written exam (80%) and ongoing assessment (10%)

Contacts

Project Leader: L. Gannoun, DIMNP, UM
Administrative Contact(s): Claudie Fabry