HAV305V Biochemistry 3 (3 ECTS)
Presentation
Description:
This TU allows students to consolidate the fundamentals of biochemistry acquired in L1 by approaching this discipline through a cross-disciplinary study of enzymes involved in cellular metabolism, particularly glycolysis. Several areas of biochemistry will be covered: the fundamentals of Michaelian enzymology, and a description of the metabolic reactions involved in glycolysis. Finally, the technical aspect will be addressed through the presentation and analysis of techniques for measuring enzyme activity and purifying, quantifying, and detecting proteins.
Objectives:
• Understand the basic concepts and principles of Michaelian enzymology and cellular energetics in order to comprehend metabolic regulation.
• Basic knowledge of the structure and metabolism of carbohydrates, particularly glycolysis.
• Understand that the "spontaneity" of reactions constituting a metabolic pathway in cells is linked to a set of intracellular factors (variable concentrations of different metabolites over time) and extracellular factors (concentration of secreted metabolites, hormonal signals, etc.).
• Locate and measure enzyme activity, measure reaction speed. Measure a metabolite.
• Understand and use common spectrophotometric dosing and analysis techniques, master common separation and purification methods.
• Understand intermolecular interactions.
Hourly volumes:
CM: 2 p.m.
TD: 2 p.m.
TP: 8 a.m.
Field: 0 hours
Required prerequisites:
L3 science or health.
Recommended prerequisites:
Nothing to report
More information
Knowledge assessment:
1 tutorial exam in the lecture hall (10%)
Written final exam (80%) and continuous assessment (10%)
Contacts
Responsible: L. Gannoun, DIMNP, UM
Administrative contact(s): Claudie Fabry